It is widely believed that there is a critical need in cancer treatment for a practical technology to allow testing of standard & experimental treatment modality directly in fresh human cancer specimen, rather than in established cell lines or
subhuman
tumor system.
In this assay cell suspension were prepared from 16 gastric, 9 colorectal tissues obtained during the operation, and we examined the chemosensitivity of 32 specimen against 11 antitumor drugs.
The results were as follows:
There was a linear relationship between the number of HeLa cells and the optical density of the formazan produce d(r=0.92).
1) Over all chemosensitive rates of 22% were noted in 32 specimen, giving an evaluation rate of 72%, the sensitivitive rate of tumors to DDP 35%, 5-FU, ADM, VLB 30%, Ara-C, VP-16, 26%, MMC, VCR 22%, Decrease in SD activity was remarkable in the
pooly
differentiated tissues compare to the well differentiated tissue for all drug except MTX. Decreae in SD activity was evident in gastric cancer tissues compared to colon cancer tissue for all drug test except 5-FU, MMC, MTX. Decrease in SD
activity
was
remarkable in metastatic lymph node compared to cancer tissue.
There were stastistical differences in 5-FU, ADM, MMC, Ara-C, VLB (p<0.05).
In summery of this experiment, the authors suggest the chemosentsitivity varied in the tissue. Histological differentiation, the origin of an organ tumor, and difference of primary or metastastic lesion are critical for determining
chemosensitivity.
The in vitro MTT assay will be a simple, rapid test and useful for mass aritiutmor drug screening.
|